|
KMID : 0624620090420100673
|
|
BMB Reports
2009 Volume.42 No. 10 p.673 ~ p.678
|
|
|
Isolation and characterization of a novel short-chain alcohol dehydrogenase gene from Panax ginseng
|
|
Kim Yu-Jin
Shim Ju-Sun
Lee Jung-Hye
Jung Dae-Young
Sun Hwa
In Jun-Gyo
Yang Deok-Chun
|
|
|
Abstract
|
|
|
|
The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoelectric point of 6.84. Homology analysis revealed that the deduced amino acid of PgADH shares a high degree of homology with the short-chain ADH proteins of other plants. Genomic DNA hybridization analysis indicated that PgADH represents a multi-gene family. The expression of PgADH under various environmental stresses was analyzed at different time points using real-time PCR. ABA, SA and especially JA (80-fold) significantly induced PgADH expression within 24 h of treatment. The positive responses of PgADH to abiotic stimuli suggest that ginseng ADH may protect against hormone-related environmental stresses.
|
|
|
KEYWORD
|
|
|
Abiotic stress, Gene expression, Ginseng, JA, Short chainalcohol dehydrogenase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|